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α tgn38 monoclonal antibody mouse  (Novus Biologicals)


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    Structured Review

    Novus Biologicals α tgn38 monoclonal antibody mouse
    α Tgn38 Monoclonal Antibody Mouse, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tgn38/TGN38+Antibody+(2F7%2E1)/pmc13008938-113-1-10
    Average 94 stars, based on 31 article reviews
    α tgn38 monoclonal antibody mouse - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Immunostaining:

    Article Title: A metabolic redox relay supports ER proinsulin export in pancreatic islet β cells
    Article Snippet: .. For immunostaining, cells were incubated overnight with antibodies raised against BiP (rabbit; gift of Christopher Nicchitta, Duke University, Durham, North Carolina, USA; 1:200), GRASP55 (rabbit, Proteintech 10598-AP, 1:500), GM130 (mouse, BD Transduction 610-823, 1:200), TGN38 (mouse, Novus Biologicals, Bio-Techne, NB300-575, 1:200), or proinsulin (mouse, MyBioSource MBS660187, 1:200) as indicated. .. Highly cross-adsorbed fluorescence-conjugated secondary antibodies were used for detection: whole IgG, donkey anti-rabbit rhodamine red-X (Jackson ImmunoResearch 711-295-152), or donkey anti-mouse Alexa Fluor 647 (Jackson ImmunoResearch 715-606-150).

    Article Title: Synchronized proinsulin trafficking reveals delayed Golgi export accompanies β-cell secretory dysfunction in rodent models of hyperglycemia
    Article Snippet: .. For immunostaining, permeabilized cells were incubated overnight with antibodies raised against cation independent-M6PR (rabbit, Proteintech 20253-1-AP), CPE (rabbit, Proteintech 13710-1-AP), GM130 (mouse, BD Transduction 610,823), GRP94 (rabbit, kind gift of Dr. Christopher Nicchita, Duke University), SA (rabbit, Rockland 100-4195), and TGN38 (mouse, Novus Biologicals NB300-575) as indicated. .. Highly cross-adsorbed fluorescent conjugated secondary antibodies (whole IgG, donkey anti-guinea pig AlexaFluor488, donkey anti-rabbit Cy3, and donkey anti-mouse AlexaFluor 647; Jackson ImmunoResearch) were used for detection.

    Article Title: Synchronized proinsulin trafficking reveals delayed Golgi export accompanies β-cell secretory dysfunction in rodent models of hyperglycemia.
    Article Snippet: .. For immunostaining, permeabilized cells were incubated overnight with antibodies raised against cation independent-M6PR (rabbit, Proteintech 20253-1-AP), CPE (rabbit, Proteintech 13710-1-AP), GM130 (mouse, BD Transduction 610,823), GRP94 (rabbit, kind gift of Dr. Christopher Nicchita, Duke University), SA (rabbit, Rockland 100-4195), and TGN38 (mouse, Novus Biologicals NB300-575) as indicated. ..

    Incubation:

    Article Title: A metabolic redox relay supports ER proinsulin export in pancreatic islet β cells
    Article Snippet: .. For immunostaining, cells were incubated overnight with antibodies raised against BiP (rabbit; gift of Christopher Nicchitta, Duke University, Durham, North Carolina, USA; 1:200), GRASP55 (rabbit, Proteintech 10598-AP, 1:500), GM130 (mouse, BD Transduction 610-823, 1:200), TGN38 (mouse, Novus Biologicals, Bio-Techne, NB300-575, 1:200), or proinsulin (mouse, MyBioSource MBS660187, 1:200) as indicated. .. Highly cross-adsorbed fluorescence-conjugated secondary antibodies were used for detection: whole IgG, donkey anti-rabbit rhodamine red-X (Jackson ImmunoResearch 711-295-152), or donkey anti-mouse Alexa Fluor 647 (Jackson ImmunoResearch 715-606-150).

    Article Title: Proinflammatory cytokines mediate pancreatic β-cell specific alterations to Golgi morphology via iNOS-dependent mitochondrial inhibition
    Article Snippet: .. Permeabilized cells were incubated overnight with antibodies raised against GM130 (mouse, BD Transduction 610,823), Golgin97 (rabbit, Proteintech 12640-1-AP), insulin (guinea pig, Fitzgerald 70R-10659), or TGN38 (mouse, Novus Biologicals NB300-575) as indicated. .. Highly cross-adsorbed fluorescent conjugated secondary antibodies (whole IgG, donkey anti-mouse AlexaFluor 647, donkey anti-rabbit Cy3, and donkey anti-guinea pig AlexaFluor488; Jackson ImmunoResearch) were used for detection.

    Article Title: Synchronized proinsulin trafficking reveals delayed Golgi export accompanies β-cell secretory dysfunction in rodent models of hyperglycemia
    Article Snippet: .. For immunostaining, permeabilized cells were incubated overnight with antibodies raised against cation independent-M6PR (rabbit, Proteintech 20253-1-AP), CPE (rabbit, Proteintech 13710-1-AP), GM130 (mouse, BD Transduction 610,823), GRP94 (rabbit, kind gift of Dr. Christopher Nicchita, Duke University), SA (rabbit, Rockland 100-4195), and TGN38 (mouse, Novus Biologicals NB300-575) as indicated. .. Highly cross-adsorbed fluorescent conjugated secondary antibodies (whole IgG, donkey anti-guinea pig AlexaFluor488, donkey anti-rabbit Cy3, and donkey anti-mouse AlexaFluor 647; Jackson ImmunoResearch) were used for detection.

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure.
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 ◦C overnight. ..

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 °C overnight. ..


    Article Title: Synchronized proinsulin trafficking reveals delayed Golgi export accompanies β-cell secretory dysfunction in rodent models of hyperglycemia.
    Article Snippet: .. For immunostaining, permeabilized cells were incubated overnight with antibodies raised against cation independent-M6PR (rabbit, Proteintech 20253-1-AP), CPE (rabbit, Proteintech 13710-1-AP), GM130 (mouse, BD Transduction 610,823), GRP94 (rabbit, kind gift of Dr. Christopher Nicchita, Duke University), SA (rabbit, Rockland 100-4195), and TGN38 (mouse, Novus Biologicals NB300-575) as indicated. ..

    Isolation:

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure.
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 ◦C overnight. ..

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 °C overnight. ..

    Cell Culture:

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure.
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 ◦C overnight. ..

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 °C overnight. ..

    Blocking Assay:

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure.
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 ◦C overnight. ..

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 °C overnight. ..

    Whole Genome Amplification:

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure.
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 ◦C overnight. ..

    Article Title: Novel Identification of Ankyrin-R in Cardiac Fibroblasts and a Potential Role in Heart Failure
    Article Snippet: .. Isolated cardiac fibroblasts were cultured on glass coverslips and fixed with 1:1 acetone:methanol for 10 min and incubated in blocking solution (PBS with 3% fish gelatin, 0.75% Triton-X 100 (10%), 1% DMSO) for 1 h. Cells were then incubated in primary antibody (custom AnkR antibody (1:300); WGA-488 5 μg/mL Thermo Fisher, W11261; TGN38 1:1000, Novus Biologicals, Centennial, CO USA NB300-575; αSMA, 1:500 Sigma-Aldrich A2547-100UL) at 4 °C overnight. ..



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